Journal: BMC pulmonary medicine

Article Title: Angiotensin-(1-7) suppresses airway inflammation and airway remodeling via inhibiting ATG5 in allergic asthma.

doi: 10.1186/s12890-023-02719-7

Figure Lengend Snippet: Fig. 1 Effects of Ang-(1–7) on ATG5 protein expression in IL-13-treated BEAS-2B and HBSMC cells. A BEAS-2B and HBSMC cells were treated with different dose (5, 10, 20, 40 µM) of Ang-(1–7) for 24 h. Cell viability was measured via a CCK-8 kit. BEAS-2B and HBSMC cells were treated with 10 ng/mL human IL-13 for 24 h, then treated with different dose (5, 10, 20 µM) of Ang-(1–7) for 24 h. Expression of ATG5 protein in BEAS-2B cells (B) and HBSMC cells (C) was measured by western blotting. *p < 0.05, **p < 0.01. ns: no significance

Article Snippet: The Ang-(1–7) (#HY-12403, MedChemExpress, China) was dissolved in 1% phosphate buffer solution (PBS), and 1% PBS was used as a control.

Techniques: Expressing, CCK-8 Assay, Western Blot

Journal: BMC pulmonary medicine

Article Title: Angiotensin-(1-7) suppresses airway inflammation and airway remodeling via inhibiting ATG5 in allergic asthma.

doi: 10.1186/s12890-023-02719-7

Figure Lengend Snippet: Fig. 2 Ang-(1–7) treatment suppressed the expression of Beclin-1 protein and secretions of IL-25 and IL-33 in the IL-13-treated BEAS-2B cells. BEAS-2B cells were transfected with control siRNA or ATG5 siRNA, and then treated with IL-13 for 24 h. The IL-13-treated cells were treated with 20 µM Ang-(1–7) for 24 h, and the PBS treatment was as a control treatment. A Cell viability in different groups was measured using a CCK-8 kit. B Expression of Beclin-1 protein was measured using Western blotting. C Secretion of IL-25 and IL-33 was measured using ELISA kits. ns: no significance. *p < 0.05, **p < 0.01

Article Snippet: The Ang-(1–7) (#HY-12403, MedChemExpress, China) was dissolved in 1% phosphate buffer solution (PBS), and 1% PBS was used as a control.

Techniques: Expressing, Transfection, Control, CCK-8 Assay, Western Blot, Enzyme-linked Immunosorbent Assay

Journal: BMC pulmonary medicine

Article Title: Angiotensin-(1-7) suppresses airway inflammation and airway remodeling via inhibiting ATG5 in allergic asthma.

doi: 10.1186/s12890-023-02719-7

Figure Lengend Snippet: Fig. 3 Ang-(1–7) treatment suppressed the expression of Beclin-1, TGF-β1 and α-SMA protein in the IL-13-treated HBSMC cells. HBSMC cells were transfected with control siRNA or ATG5 siRNA, and then treated with IL-13 for 24 h. The IL-13-treated cells were treated with 20 µM Ang-(1–7) for 24 h, and the PBS treatment was as a control treatment. A Cell viability was measured using a CCK-8 kit. B Expression of Beclin-1 protein was measured using Western blotting. C Expression of TGF-β1 and α-SMA protein was measured using Western blotting. ns: no significance. *p < 0.05, **p < 0.01

Article Snippet: The Ang-(1–7) (#HY-12403, MedChemExpress, China) was dissolved in 1% phosphate buffer solution (PBS), and 1% PBS was used as a control.

Techniques: Expressing, Transfection, Control, CCK-8 Assay, Western Blot

Journal: BMC pulmonary medicine

Article Title: Angiotensin-(1-7) suppresses airway inflammation and airway remodeling via inhibiting ATG5 in allergic asthma.

doi: 10.1186/s12890-023-02719-7

Figure Lengend Snippet: Fig. 4 Ang-(1–7) suppressed the results of ATG5 overexpression in IL-13-induced BEAS-2B cells. The BEAS-2B cells were transfected with ATG5 cDNA or vector control, and treated with IL-13 for 24 h. The ATG5 cDNA-transfected or vector control-transfected cells were treated with 20 µM Ang-(1–7) treatment. A Expression of Beclin-1 protein was measured using Western blotting. B Secretion of IL-25 and IL-33 was measured using ELISA kits. **p < 0.01

Article Snippet: The Ang-(1–7) (#HY-12403, MedChemExpress, China) was dissolved in 1% phosphate buffer solution (PBS), and 1% PBS was used as a control.

Techniques: Over Expression, Transfection, Plasmid Preparation, Control, Expressing, Western Blot, Enzyme-linked Immunosorbent Assay

Journal: BMC pulmonary medicine

Article Title: Angiotensin-(1-7) suppresses airway inflammation and airway remodeling via inhibiting ATG5 in allergic asthma.

doi: 10.1186/s12890-023-02719-7

Figure Lengend Snippet: Fig. 5 Ang-(1–7) suppressed the results of ATG5 overexpression in IL-13-induced HBSMC cells. The HBSMC cells were transfected with ATG5 cDNA or vector control, and treated with IL-13 for 24 h. The ATG5 cDNA-transfected or vector control-treated cells were treated with 20 µM Ang-(1–7) treatment. A Expression of Beclin-1, TGF-β1 and α-SMA protein was measured using Western blotting. B Relative expression of Beclin-1 protein was normalized by GAPDH. C Relative expression of TGF-β1 protein was normalized by GAPDH. D Relative expression of α-SMA protein was normalized by GAPDH. *p < 0.05, **p < 0.01

Article Snippet: The Ang-(1–7) (#HY-12403, MedChemExpress, China) was dissolved in 1% phosphate buffer solution (PBS), and 1% PBS was used as a control.

Techniques: Over Expression, Transfection, Plasmid Preparation, Control, Expressing, Western Blot

Journal: BMC pulmonary medicine

Article Title: Angiotensin-(1-7) suppresses airway inflammation and airway remodeling via inhibiting ATG5 in allergic asthma.

doi: 10.1186/s12890-023-02719-7

Figure Lengend Snippet: Fig. 6 Ang-(1–7) treatment or ATG5 knockout suppressed airway inflammation in OVA-challenged mice. A The diagram of animal treatments. An allergic asthma model was induced by OVA in WT mice and ATG5.−/− mice. The OVA-induced WT mice were treated with 30 µg/kg/d Ang-(1–7) for 10 days after sensitization at day 8. B Airway resistance to methacholine were measured. C Levels of IL-4, IL-5 and IL-33 in BALFs were measured using ELISA kits. D Observation of pathological changes by H&E staining. Area of inflammatory infiltration in airway was analyzed using ImageJ software. **p < 0.01

Article Snippet: The Ang-(1–7) (#HY-12403, MedChemExpress, China) was dissolved in 1% phosphate buffer solution (PBS), and 1% PBS was used as a control.

Techniques: Knock-Out, Enzyme-linked Immunosorbent Assay, Staining, Software

Journal: BMC pulmonary medicine

Article Title: Angiotensin-(1-7) suppresses airway inflammation and airway remodeling via inhibiting ATG5 in allergic asthma.

doi: 10.1186/s12890-023-02719-7

Figure Lengend Snippet: Fig. 7 Ang-(1–7) treatment or ATG5 knockout suppressed airway remodeling in by OVA-challenged mice. Expression of α-SMA (A) and TGF-β1 (B) in airway was analyzed by immunohistochemical analysis. Expression of results was analyzed by ImageJ software. *p < 0.05, **p < 0.01

Article Snippet: The Ang-(1–7) (#HY-12403, MedChemExpress, China) was dissolved in 1% phosphate buffer solution (PBS), and 1% PBS was used as a control.

Techniques: Knock-Out, Expressing, Immunohistochemical staining, Software

Journal: BMC pulmonary medicine

Article Title: Angiotensin-(1-7) suppresses airway inflammation and airway remodeling via inhibiting ATG5 in allergic asthma.

doi: 10.1186/s12890-023-02719-7

Figure Lengend Snippet: Fig. 8 Ang-(1–7) treatment or ATG5 knockout suppressed airway autophagy in OVA-challenged mice. A Expression of LC3B in airway was evaluated by immunohistochemical analysis. B Expression of Beclin-1 in airway was evaluated by western blotting. *p < 0.05, **p < 0.01

Article Snippet: The Ang-(1–7) (#HY-12403, MedChemExpress, China) was dissolved in 1% phosphate buffer solution (PBS), and 1% PBS was used as a control.

Techniques: Knock-Out, Expressing, Immunohistochemical staining, Western Blot

Journal: Molecular Medicine Reports

Article Title: BPA disrupts 17-estradiol-mediated hepatic protection against ischemia/reperfusion injury in rat liver by upregulating the Ang II/AT1R signaling pathway

doi: 10.3892/mmr.2020.11072

Figure Lengend Snippet: Effects of BPA, E2 and LOS on Ang II expression. (A) Serum and (B) hepatic Ang II expression levels at 24 h post-reperfusion. *P<0.05 vs. Sham, Sham + BPA or I/R + E2. BPA, bisphenol A; E2, 17β-estradiol; LOS, losartan; Ang II, angiotensin II; I/R, ischemia/reperfusion.

Article Snippet: Serum and hepatic Ang II levels were measured using an Ang II ELISA kit (cat. no. ABIN416068; Uscn Life Science Inc.), according to the manufacturer's protocol.

Techniques: Expressing